Course curriculum
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1
Module 5 Instructions
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Content
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Instructions
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Suggested timeline for experiments in the OrganoPlate Graft
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2
Chapter 5A. General Materials
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Objective
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1. Pipettes
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2. Mimetas Materials
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3
Chapter 5B. ECM Preparation
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Objective
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Storage information Collagen-I
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Preparation of the Collagen-I working solution
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[Trainings video] Collagen-I preparation
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Test Your Knowledge!
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4
Chapter 5C. ECM Loading
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Objective
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Collagen-I loading
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[Trainings video] ECM loading in the OrganoPlate® Graft
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5
Chapter 5D. Cell Seeding
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Objective
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Cells for tubules formation
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Preparation of the desired cell type
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Cell seeding
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[Trainings video] Cell Seeding in the OrganoPlate® Graft
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6
Chapter 5E. Medium Change + Inducing Sprouts formation
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Objective
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Medium change
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Induction of vascular bed formation/ endothelial sprouting
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[Trainings video] Medium change and inducing angiogenic sprout formation in the OrganoPlate® Graft
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7
Chapter 5F. Tissue Placement
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Objective
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Tissue for placement in the OrganoPlate® Graft
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Tissue placement of tissue <1mm (no cutting and ECM embedding)
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Tissue placement on tissue >1mm (with cutting and ECM embedding)
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[Trainings video] Tissue Placement in the OrganoPlate® Graft
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Correcting microtissue positioning in the OrganoPlate® Graft
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[Trainings video] Correcting microtissue positioning in the Graft chamber
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8
FAQ
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General OrganoPlate® Questions
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ECM loading/ECM gel related Questions
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Cell seeding/ Cell type related Questions
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Medium Change Questions
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OrganoFlow®/Perfusion Questions
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OrganoPlate® Graft specific Questions
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