Course curriculum

  • 1

    Module 5 Instructions

    • Content

    • Instructions

    • Suggested timeline for experiments in the OrganoPlate Graft
  • 2

    Chapter 5A. General Materials

    • Objective

    • 1. Pipettes

    • 2. Mimetas Materials
  • 3

    Chapter 5B. ECM Preparation

    • Objective

    • Storage information Collagen-I

    • Preparation of the Collagen-I working solution

    • [Trainings video] Collagen-I preparation

    • Test Your Knowledge!
  • 4

    Chapter 5C. ECM Loading

    • Objective

    • Collagen-I loading

    • [Trainings video] ECM loading in the OrganoPlate® Graft
  • 5

    Chapter 5D. Cell Seeding

    • Objective

    • Cells for tubules formation

    • Preparation of the desired cell type

    • Cell seeding

    • [Trainings video] Cell Seeding in the OrganoPlate® Graft
  • 6

    Chapter 5E. Medium Change + Inducing Sprouts formation

    • Objective

    • Medium change

    • Induction of vascular bed formation/ endothelial sprouting

    • [Trainings video] Medium change and inducing angiogenic sprout formation in the OrganoPlate® Graft
  • 7

    Chapter 5F. Tissue Placement

    • Objective

    • Tissue for placement in the OrganoPlate® Graft

    • Tissue placement of tissue <1mm (no cutting and ECM embedding)

    • Tissue placement on tissue >1mm (with cutting and ECM embedding)

    • [Trainings video] Tissue Placement in the OrganoPlate® Graft

    • Correcting microtissue positioning in the OrganoPlate® Graft

    • [Trainings video] Correcting microtissue positioning in the Graft chamber
  • 8

    FAQ

    • General OrganoPlate® Questions

    • ECM loading/ECM gel related Questions

    • Cell seeding/ Cell type related Questions

    • Medium Change Questions

    • OrganoFlow®/Perfusion Questions

    • OrganoPlate® Graft specific Questions