Course curriculum

  • 1

    1. Introduction to OrganoAccess and the OrganoPlate® Graft 32 UF

    • 1.1 Introduction and Goals

    • 1.2 Technology Fundamentals

    • 1.3 Introduction seminar
  • 2

    Materials

    • 2.1 Materials required
  • 3

    3. Procedure: Vascular bed formation

    • 3.1 Procedure Overview

    • 3.2 Thawing of cells

    • 3.3 Cell Maintenance

    • 3.4 Cell Harvesting

    • 3.5 Gel Loading

    • 3.6 Coating the Perfusion Channels

    • 3.7 Cell Seeding

    • 3.8 Initiating Perfusion

    • Filling the evaporation barrier wells

  • 4

    4. Assays on the OrganoPlate Graft UniFlow

    • 4.1 Perfusion assay

    • 4.2 Immunofluorescent Staining
  • 5

    Troublehooting

    • 5.1 Troubleshooting Tips