Course curriculum

  • 1

    Module 3 Instructions

    • Content

    • Instructions

    • Suggested timeline of experiments in the OrganoPlate 3-lane 40
  • 2

    Chapter 3A. General Materials

    • Objective

    • 1.Pipettes

    • 2. Mimetas Materials
  • 3

    Chapter 3B. ECM Preparation

    • Objective

    • Storage information Collagen-I

    • 1. Preparation of the OrganoPlate®

    • 2. Preparation of the Collagen-I working solution

    • [Trainings video] Collagen-I preparation

    • Test Your Knowledge!
  • 4

    Chapter 3C. ECM Loading

    • Objective

    • Collagen-I loading

    • [Trainings video] ECM loading in the OrganoPlate® 3-lane 40

    • Test Your Knowledge!
  • 5

    Chapter 3D. Cell Seeding

    • Objective

    • Cells for seeding in the OrganoPlate®

    • Preparation of the desired cell type

    • Cell seeding of endothelial cells

    • Cell seeding of Caco-2 cells alone or in combination with HT29 cellls

    • [Trainings video] Cell Seeding in the OrganoPlate® 3-lane 40
  • 6

    Chapter 3E. Medium Change

    • Objective

    • Medium change with medium in top and bottom channel - Caco-2 cells

    • Medium change with medium only in the top channel - endothelial/ epithelial cultures

    • [Trainings video] Medium change in the OrganoPlate® 3-lane 40
  • 7

    FAQ

    • General OrganoPlate® Questions

    • ECM loading/ECM gel related Questions

    • Cell seeding/ Cell type related Questions

    • Medium Change Questions

    • OrganoFlow®/Perfusion Questions