Course curriculum

  • 1

    Module 3 Instructions

    • Content

    • Instructions
  • 2

    Chapter 3A. General Materials

    • Objective

    • 1.Pipettes

    • 2. Mimetas Materials
  • 3

    Chapter 3B. Preparation for Medium Change

    • Unpacking the OrganoReady® plate after delivery

    • Preparation of the OrganoReady® plate for the media change
  • 4

    Chapter 3C. Medium Change

    • Objective

    • Medium change in the OrganoReady® plate

    • Preparation of the MicroClime® lid and restarting perfusion flow
  • 5

    Chapter 3D. Seeding neuronal cells in the OrganoReady BBB HBMEC plate

    • Objective

    • Preparation of neuronal cells

    • Neuronal cell seeding in the OrganoReady® BBB HBMEC plate

  • 6

    Chapter 3E. Small molecule transport in the OrganoReady plate

    • Objective

    • Compound preparation before the assay

    • Assessing small molecule compound transport in the OrganoReady® BBB HBMEC plate

  • 7

    FAQ

    • General OrganoPlate® Questions

    • Medium Change Questions

    • OrganoFlow®/Perfusion Questions

    • Cell seeding/ cell type related questions