Course curriculum

  • 1

    Module 3 Instructions

    • Content

    • Instructions

    • Suggested timeline for experiments in the OrganoReady Collagen 3-lane 64 plate
  • 2

    Chapter 3A. General Materials

    • Objective

    • 1.Pipettes

    • 2. Mimetas Materials
  • 3

    Chapter 3B. Preparation for Tubule seeding in the OrganoReady plate

    • Storing and unpacking the OrganoReady® plate after delivery
  • 4

    Chapter 3C. Wet channel seeding to grow tubular structures in the OrganoReady plate

    • Objective

    • Preparation of the desired cell type

    • Performing wet channel seeding in the OrganoReady® Collagen plate - endothelial/ epithelial cells

    • Performing wet channel seeding in the OrganoReady® Collagen plate - Caco-2 cells or Caco-2/HT29 co-cultures

    • Preparation of the MicroClime® lid and restarting perfusion flow
  • 5

    Chapter 3D. Medium change in the OrganoReady 3-lane 64 plate

    • Objective

    • Medium change with medium only in the right perfusion channel

    • Medium change with medium in both perfusion channels e.g. for Caco2 cultures

    • [Trainings video] Medium change in the OrganoPlate® 3-lane 64
  • 6

    FAQ

    • General OrganoPlate® Questions

    • Medium Change Questions

    • OrganoFlow®/Perfusion Questions

    • Cell Seeding/ Cell type related questions