Course curriculum
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1
Module 3 Instructions
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Content
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Instructions
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Suggested timeline for experiments in the OrganoReady Collagen 3-lane 64 plate
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2
Chapter 3A. General Materials
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Objective
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1.Pipettes
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2. Mimetas Materials
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3
Chapter 3B. Preparation for Tubule seeding in the OrganoReady plate
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Storing and unpacking the OrganoReady® plate after delivery
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4
Chapter 3C. Wet channel seeding to grow tubular structures in the OrganoReady plate
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Objective
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Preparation of the desired cell type
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Performing wet channel seeding in the OrganoReady® Collagen plate - endothelial/ epithelial cells
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Performing wet channel seeding in the OrganoReady® Collagen plate - Caco-2 cells or Caco-2/HT29 co-cultures
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Preparation of the MicroClime® lid and restarting perfusion flow
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5
Chapter 3D. Medium change in the OrganoReady 3-lane 64 plate
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Objective
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Medium change with medium only in the right perfusion channel
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Medium change with medium in both perfusion channels e.g. for Caco2 cultures
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[Trainings video] Medium change in the OrganoPlate® 3-lane 64
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6
FAQ
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General OrganoPlate® Questions
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Medium Change Questions
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OrganoFlow®/Perfusion Questions
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Cell Seeding/ Cell type related questions
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